1α,25-Dihydroxyvitamin D3 Ameliorates Seawater Aspiration-Induced Acute Lung Injury via NF-κB and RhoA/Rho Kinase Pathways
Figure 4
Effects of calcitriol on NF-κB p65 activation and RhoA/ROCK pathway in A549 cells.
After 4 h seawater treatment, proteins were obtained for western blot. The ratios of pNF-κB versus NF-κB, GTP-RhoA versus total-RhoA and p-MYPT1 versus MYPT1 were obtained for each group to examine the pNF-κB, GTP-RhoA and p-MYPT1 content. NG: normal group; SG: seawater group; CG: 10−6 M calcitriol group; DG: dexamethasone group. n = 6, ***P<0.001 versus group NG, ## P<0.01, ### P<0.001 versus group SG, ° P<0.05 versus group DG.