Dual Transgene Expression in Murine Cerebellar Purkinje Neurons by Viral Transduction In Vivo
Figure 1
Schematic representation of lentiviral constructs.
A–B. Schematic of the lentiviral constructs used in this study. Arrows represent mammalian transcriptional start sites. In all vectors, the 3′-LTR contains a deletion in the U3 region which renders the virus self-inactivating (SIN 3′-UTR) and replication incompetent. A. The MND-tdTomato lentiviral vector contains a microRNA (miR30) and chloramphenicol resistance gene (CMR) in the 3′-UTR of the tdTomato fluorescent reporter gene. B. The internal promoters are MND, modified MoMuLV LTR containing myeloproliferative sarcoma virus enhancer; MSCV, murine stem cell virus LTR; UBC, Ubiquitin C promoter; and PGK, human phosphoglyercerate kinase promoter. Ψ: packaging signal; RRE, REV response element; cPPT, central polypurine tract; EGFP, enhanced green fluorescent protein; IRES, internal ribosome entry site; WPRE, woodchuck hepatitis virus posttranscriptional regulatory element; LTR, long terminal repeat.