Macrophage Phenotype Is Associated with Disease Severity in Preterm Infants with Chronic Lung Disease
Figure 2
Flow cytometry strategy for identifying functionally polarised macrophage populations in BALF.
Infants were ventilated and lavaged within 24-CD15 (A), anti-CD14 (B), anti-CD16 (C), anti-HLADR (D), anti-CD36 (E) or matched isotype antibodies and analysed by flow cytometry. Staining profiles showing isotype controls in blue and target antibody in red. CD14/CD16 staining profile shown in F. Positively stained events are higlighted in FSC and SSC plots in blue for HLADR (G), CD36 (H), CD14/HLADR dual stained (I) and CD14/CD36 dual stained (J). FSC/SSC profiles of CD14+ and: HLADR− (K), HLADR+ (L), CD36− (M) and CD36+ (N) events. Plots illustrate representative data from 3 individual subjects.