Comparative 2D-DIGE Proteomic Analysis of Bovine Mammary Epithelial Cells during Lactation Reveals Protein Signatures for Lactation Persistency and Milk Yield
Figure 12
Validation of 2D-DIGE data by quantitative PCR and compared with the 2D-DIGE data.
Quantitative PCR analysis was performed for the genes shown in the figure and compared its fold expression change in relation to the 2D-DIGE data. A. The expression profile of proteins at peak and late stage of lactation were represented in comparison to early stage that was normalized to 1. B. The expression profile of proteins of Hy and KF samples were represented in comparison to Ly samples that was normalized to 1.