Skip to main content
Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

Tomato NAC Transcription Factor SlSRN1 Positively Regulates Defense Response against Biotic Stress but Negatively Regulates Abiotic Stress Response

Figure 4

SlSRN1 positively regulates resistance response against B. cinerea.

A–C. Silencing of SlSRN1 led to enhanced susceptibility to B. cinerea. A. Silencing efficiency in TRV-SlSRN1-infiltrated tomato plants. Two-week-old seedlings were infiltrated with agrobacteria carrying TRV-SlSRN1 or TRV-GUS and leaf samples were collected 4 weeks after VIGS treatment. The transcript of SlSRN1 was analysed by qRT-PCR. B and C. Disease phenotype and lesion size on detached leaves of TRV-SlSRN1 or TRV-GUS-infiltrated plants after inoculation with B. cinerea, respectively. D-E. Transient overexpression of SlSRN1 resulted in increased resistance to B. cinerea. D. Expression of SlSRN1 in pFGC-SlSRN1- or pFGC-eGFP-infiltrated plants. Leaves of 3-week-old seedlings were infiltrated with agrobacteria carrying pFGC-SlSRN1- or pFGC-eGFP vector and leaf samples were collected 3 days after infiltration. E and F. Disease phenotype and lesion size on detached leaves of pFGC-SlSRN1- or pFGC-eGFP-infiltrated plants after inoculation with B. cinerea, respectively. Data presented are the means ± SD from three independent experiments and different letters above the columns indicate significant differences at p<0.05 level.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0102067.g004