Increasing Maternal or Post-Weaning Folic Acid Alters Gene Expression and Moderately Changes Behavior in the Offspring
Figure 2
A: Quantitative RT-PCR confirmation of microarray data.
Relative expression of the Cpn2, Zfp353, Htr4, Nkx6-3 and Xist transcripts in the cerebral hemispheres of male newborn pups, from mothers supplemented with FA at 4 mg/kg of diet. The results were normalized to Hprt transcript expression, and expressed as relative values in comparison to corresponding transcripts from newborn pups with FA at 0.4 mg/kg diet. Results represent means ± S.D; asterisks denote statistically significant change (***P<0.001, **P<0.01 and *P<0.05). B): Quantitative RT PCR confirmation of microarray data. Relative expression of the Vgll2, Leprel1, Nfix and Slc17a7 transcripts from the cerebral hemispheres of female newborn pups, from mothers supplemented with FA at 4 mg/kg of diet. The results were normalized to Gapdh transcript expression, and were expressed as relative values in comparison to corresponding transcripts from newborn pups with FA at 0.4 mg/kg diet. Results represent means ± S.D; asterisks denote statistically significant change (***P<0.001, **P<0.01 and *P<0.05).