Identification and Purification of Human Induced Pluripotent Stem Cell-Derived Atrial-Like Cardiomyocytes Based on Sarcolipin Expression
Figure 4
Redhigh cells display electrophysiological properties similar to atrial cardiomyocytes.
(a) Representative triggered action potential traces recorded from redhigh and redlow cells. (b) Quantification of APD50 and APD90 for redhigh (n = 15) and redlow (n = 10) cells (***p<0.001, *p<0.05). (c–e) Expression of depolarization-activated potassium currents. (c) Upper panel: representative instantaneous outward currents. Lower panel: representative sustained potassium currents. (d) Comparison of peak instantaneous outward currents between redhigh (n = 7) and redlow (n = 8) cells. (e) Comparison of I–V curves of sustained potassium currents between redhigh and redlow cells (***p<0.001). (f) qPCR for gene expression of the IKur subunit KCNA5 and IKAch subunit KCNJ3, revealing increased expression in redhigh cells (*p<0.05, ****p<0.0001). All genes normalized to expression of GAPDH and relative to gene expression in EBs.