Ectosomes: A New Mechanism for Non-Exosomal Secretion of Tau Protein
Figure 5
EcEF (upper panel) and ExEF (lower panel) obtained from stable N1E-115 overexpressing h1N4R cells (left part of the immunoblots) or from naive N1E-115 (right part of the immunoblots) were incubated with growing concentrations of NaCl (0.01 to 0.5M) before western blotting analyses using a total Tau N-ter antibody and a flotillin-1 antibody. EcEF and ExEF obtained from naive N1E-115 were previously incubated with recombinant h1N4R Tau. Cell lysates (CL) from both cell lines and recombinant h1N4R Tau were used as controls.