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Carbon Monoxide Induces Heme Oxygenase-1 to Modulate STAT3 Activation in Endothelial Cells via S-Glutathionylation

Figure 3

CO increases oxidative stress in ECs.

A. and B. ECs were exposed to 25 µM TCDC or 5mM MC for 1, 3, 6 and 12 hours with or without pretreated 500U catalase for 30 min and the intracellular ROS levels were then measured. The results shown are the mean ± SEM. *P<0.05 compared with untreated ECs. C. and D. ECs were subjected to similar treatments prior to the measurement of intracellular ROS levels. The folds of lucigenin-amplified chemiluminescence are shown as the mean ± SEM. Results are presented as the mean ± SEM (n = 3). *P<0.05 compared with untreated ECs.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0100677.g003