Transient Cholesterol Effects on Nicotinic Acetylcholine Receptor Cell-Surface Mobility
Figure 5
Motional regimes and diffusion coefficient of fluorescent BTX-labeled AChR particles.
a) Distribution of diffusion coefficients for all trajectories for control fluorescent BTX-labeled samples (red) and samples treated with 10 mM CDx (green), respectively. The shaded inset (top left corner, same applies to Fig. 7a) indicates the upper limit of the microscopic diffusion coefficient D2–4, which in turn determines whether a trajectory can be classified as stationary or not. A stationary trajectory can be considered a special case of the Brownian simple and restricted motions, in which the particle does not diffuse beyond a critical value (4.6×10−12 cm2 s−1 [28]). As is apparent in Figure 7e, very few particles fall within this category, with little if any mobility. b) Proportion of different types of motion undergone by all trajectories (S-simple; D-directed; R-restricted or confined; U-undetermined). The color code represents different experiments: control fluorescent BTX-labeled samples (blue), samples treated with 10 mM CDx for 15 min (red) and for 40 min (green), respectively. c) Representative percentages of types of motion corresponding to the histograms in Figure 5b.