Interferon-Induced Genes of the Expanded IFIT Family Show Conserved Antiviral Activities in Non-Mammalian Species
Figure 4
In vitro effect of IFNs on IFITs expression in ZF4 cells and kidney primary cell cultures.
A. Expression levels of IFIT genes in ZF4 cells after 4 and 24 hours of stimulation with supernatants from transfected HEK-293 cells with plasmids containing sequences for zf-IFNΦ1, zf-IFNΦ2 and zf-IFNΦ3. B. Kidney primary cell cultures after 4 and 24 hours of stimulation with supernatants from transfected HEK-293 cells with plasmids containing sequences for zf-IFNΦ1, zf-IFNΦ2 and zf-IFNΦ3. After 4 and 24 hours of stimulation, RNA was extracted and the cDNA was synthesized. Analysis of the gene expression was performed through real-time PCR, using 18 S ribosomal RNA as a housekeeping gene. The expression level of each gene was expressed as fold-change with respect to the empty plasmid group. The data are represented as the mean ± standard error of three independent samples. Significant differences among cells transfected with IFNΦ plasmids and empty plasmid were displayed as ***(0.0001<p<0.001), **(0.001<p<0.01) or *(0.01<p<0.05).