FtsHi4 Is Essential for Embryogenesis Due to Its Influence on Chloroplast Development in Arabidopsis
Figure 7
Defects in the PSII complex of the RNAi-Ftshi4 mutant block energy transfer within PSII.
A, The chlorophyll concentrations of wild-type and RNAi-FtsHi4 mutant cotyledons and true leaves. B, Immunoblot analyses for the accumulation of D1, D2, CP43, CP47, PsbO, and Cyt f proteins in wild-type and RNAi-FtsHi4 mutant cotyledons and true leaves. The thylakoid membrane proteins were fractionated by SDS-urea-PAGE, and the blots were probed using antibodies raised against D1, D2, CP43, CP47, PsbO, or Cyt f, respectively.