Long Term Effect of Curcumin in Regulation of Glycolytic Pathway and Angiogenesis via Modulation of Stress Activated Genes in Prevention of Cancer
Figure 8
Expression and activity of MMPs.
Effect of curcumin on mRNA expression and protease activity of MMP 9 and 2 in liver of lymphoma bearing mice (A) RT-PCR of MMP9, MMP2 and β-actin genes, (B) Densitometric scanning of MMP9 and MMP2 genes after normalization with β-actin, (C) Gelatin Zymography showing protease activity of MMP9 and MMP2, (D) Densitometric scanning of the activity band of MMP9 and MMP2. Livers of all six animals of each group were pooled separately and used for extraction of total RNA and proteins at non denaturing condition. Data represent mean ± S.E.M. #p<0.05 and ##<0.01 compared to N group, *p<0.05 and **p<0.01 compared to DL+DMSO group respectively. Cur is curcumin, M is 100 bp marker and bw is body weight. N, DL, DL+DMSO, DLT50, DLT100 and DLT150 represents normal, Dalton's lymphoma bearing, Dalton's lymphoma bearing mice treated with DMSO and Dalton's lymphoma bearing mice treated with 50, 100 and 150 mg curcumin/kg body weight dissolved in DMSO respectively.