CoCl2, a Mimic of Hypoxia, Induces Formation of Polyploid Giant Cells with Stem Characteristics in Colon Cancer
Figure 6
PGCCs enrichment in colon cancer cells increases colonosphere formation capability.
After enrichment in PGCCs by treatment for 48 µM CoCl2, surviving cells were cultured at clonal density with serum free medium in low-adherence plates and the number of primary formed colonospheres was evaluated after seven days. Primary colonospheres were disaggregated and the resulting cell population was cultured again at clonal density for the formation of secondary colonospheres. Graphics show the numbers of primary (grey bars) and secondary (black bars) colonospheres formed by HCT-116 and Caco-2 cells after each treatment. The data represent the mean ± SD of three independent cultures (*p<0,05, compared to control).