Human Prominin-1 (CD133) Is Detected in Both Neoplastic and Non-Neoplastic Salivary Gland Diseases and Released into Saliva in a Ubiquitinated Form
Figure 5
Immuno-detection of prominin-1 in tumor-surrounding tissues and sialadenitis.
Sections of non-neoplastic peritumoral tissues of PA (A, B, C, D), MEC (E, F) and SA from obstructive reasons of early (G, I, J) and later (H) stages, respectively, were immunolabeled with 80B258 (A–C, E, G–I) or AC133 (D, F, J) mAb directed against prominin-1 prior to hematoxylin counterstaining. Sets of C/D, and E/F and I/J images show corresponding areas in consecutive sections. Boxed areas (C1–F1, I1, J1) are displayed at higher magnification. Non-neoplastic tissue is delimited from tumor (T; A–F) by dashed line. Black asterisks indicate inflammatory regions (A, B, H–J). Green, red and grey dashed lines indicate normal (A, G), degenerating (G) or atrophying (G) acini containing narrow, dilated or non-recognizable intercellular canaliculi (G, labeled with corresponding color arrowhead), exhibiting weak, strong or no 80B258 immunoreactivity, respectively. Orange arrowheads show atrophic secretory endpieces (B, G). Violet, black and blue lines indicate normal (A, G), dilated (B, G, H) and newly formed (H) intercalated ducts, respectively, with smooth or in latter case irregular lumen. Black and blue arrowheads indicate apical (B, C1–F1, G, H, I1, J1) and apicolateral (B, H) cell membrane immunoreactivity with 80B258 (B, C1, E1, G, H, I1) or AC133 (D1, F1, J1) mAb. Ad, adipocytes, CT, connective tissue. Histopathological characteristics of individual cases of PA: #9 (A) and #5 (B, C, D), MEC: #4 (E, F), and SA: #2 (G, I, J) and #1 (H) are summarized in Table S1. Scale bars 50 µm.