SJL Mice Infected with Acanthamoeba castellanii Develop Central Nervous System Autoimmunity through the Generation of Cross-Reactive T Cells for Myelin Antigens
Figure 5
Lymphocytes from ACA-infected mice induce CNS inflammation in naïve mice.
(A) Con-A-stimulated cells. Groups of SJL mice were infected with ACA (1×103) and the animals were killed 15 days postinfection, and lymphocytes were prepared. Cells were stimulated with Con-A (1 µg/ml) for two days; viable cells were harvested and 100×106 cells were injected i.p. into naïve mice. Brains and spinal cords were harvested on day 30 posttransfer, and the tissues were processed for CNS inflammation by H and E staining. Arrows indicate perivascular cuffing. Original magnification, ×400 (bar = 20 µm). (B) PLP 139–151-stimulated cells. Lymphocytes harvested as above were stimulated with PLP 139–151 (20 µg/ml) for two days and the cultures were maintained in IL-2 medium. Viable cells were harvested on day 5 poststimulation and 50×106 cells were injected i.p. into naïve mice. Brains and spinal cords were harvested on day 45 posttransfer and processed for evaluation of CNS inflammation by H and E staining. Arrows indicate perivascular cuffing. Original magnification, ×400 (bar = 20 µm).