Transgenic Tobacco Overexpressing Brassica juncea HMG-CoA Synthase 1 Shows Increased Plant Growth, Pod Size and Seed Yield
Figure 3
Comparison in growth between tobacco HMGS-OE seedlings/plants and vector-transformed control.
(A) Seedlings 14-d post-germination. The vector-transformed control is labelled “pSa13”, two independent lines of OE-wtBjHMGS1 plants are labelled “401” (two representative seedlings of this OE construct were shown) and “402” (three representative seedlings of this OE construct were shown) and two independent lines of OE-S359A plants are labelled “603” (two representative seedlings of this OE construct were shown) and “606” (three representative seedlings of this OE construct were shown). Bar = 1 cm. (B) Root length measurements of 14-d-old seedlings showed that tobacco HMGS-OE roots grow faster than the vector (pSa13)-transformed control. Values are mean ±SD (n = 30); Bars are SD. (C) Dry weight determination of 14-d-old seedlings shows that tobacco HMGS-OEs possess a higher mass than the vector-transformed control. Values are mean ± SD (n = 30); Bars are SD. (D) Representative greenhouse-grown plants photographed 80-d after germination. OE plants are labelled OE-wtBjHMGS1 and OE-S359A. Two independent lines of OE-wtBjHMGS1 plants, “401” (upper) and “402” (lower) and two independent lines of OE-S359A plants, “603” (upper) and “606” (lower) are shown. Bar = 10 cm. (E) Statistical analysis on height of 80-d-old transgenic plants. Values are mean ±SD (n = 6); Bars are SD; H, higher than control; a indicates significant difference between HMGS-OE and the vector (pSa13)-transformed control (P<0.01 by the Student's t-test); b indicates significant difference between OE-wtBjHMGS1 and OE-S359A (P<0.01 by the Student's t-test). pSa13, vector-transformed control; two independent lines of OE-wtBjHMGS1 (“401” and “402”) and two independent lines of OE-S359A (“603” and “606”) were used for growth rate measurement.