Abnormal Brain Iron Metabolism in Irp2 Deficient Mice Is Associated with Mild Neurological and Behavioral Impairments
Figure 1
Expression of iron homeostasis proteins in Irp2−/− and WT mice.
A) Western blot analysis of tissue extracts from male Irp2−/− and WT mice (n = 3 mice/genotype) using antibodies to detect Irp2, Irp1, ferritin (Ftl1), TfR1 and β-actin (loading control). A non-specific band migrating near Irp2 is observed in some Irp2−/− lysates. B) Irp1 and Irp2 RNA-binding activity in lysates was assayed by RNA electrophoretic mobility shift assay using a 32P-labeled ferritin IRE as a probe. 0.5% β-mercaptoethanol was added to samples to assay total RNA-binding activity.