Mesenchymal Stem Cells Derived from Human Exfoliated Deciduous Teeth (SHEDs) Induce Immune Modulatory Profile in Monocyte-Derived Dendritic Cells
Figure 4
SHEDs induce ratio-dependent BDCA1 and CD40 decrease in LPS activated moDCs.
During seven days under differentiation stimulus moDCs were matured in presence of SHEDs during the last two days of culture. The 1∶10 proportion group, since day zero, showed the most evident immune modulation by SHEDs on mDCs. Differentiation marker BDCA-1 was attenuated and co-stimulatory marker CD40, expressed on moDCs matured, was attenuated too. The characteristic molecules of them were analyzed by RFI (Relative Fluorescence Intensity). RFI presented here is a ratio between median fluorescence intensity (MFI) from experimental groups and MFI from control group (mDCs without SHEDs). mDCs – differentiated and mature DCs; d0– DCs co-cultured with SHEDs since day zero.; d5 - mDCs co-cultured with SHEDs from day five; *p≤0,05 and **p≤0,01 for the groups that showed significant differences; n = 4.