The Thoc1 Encoded Ribonucleoprotein Is Required for Myeloid Progenitor Cell Homeostasis in the Adult Mouse
Figure 3
Myeloid progenitor cells are affected by Thoc1 deficiency.
A) Bone marrow was recovered from tamoxifen treated Thoc1F/F:Rosa26CreERT2 (F/F) or Thoc1+/+:Rosa26CreERT2 (+/+) mice and the number of viable cells counted by flow cytometry. Each data point is from a different mouse with bars representing the genotype mean. Differences between genotypes are significant (t-test P = 0.01). B) An equal number of viable bone marrow cells isolated in A) were cultured in methylcellulose to assess colony forming potential. Each data point shows the number of colonies generated with samples from a different mouse with bars representing the mean. Differences between genotypes are significant (t-test P = 0.0006). C) Pre-granulocyte macrophage progenitors (Pre-GMP), granulocyte macrophage progenitors (GMP), pre-megakaryocyte erythroid progenitors (Pre-MegE), and erythroid progenitors (Pre-CFU-E) were counted in the bone marrow from A) using immunophenotyping and flow cytometry. Each data point is from a different mouse with bars representing the mean. Significant differences (t-test P<0.01) between genotypes are noted by *. D) Data from C) is plotted as a percentage of total viable bone marrow cells analyzed. Significant differences (t-test P<0.02) between genotypes are noted by *. E) A schematic outlining a simplified view of hematopoiesis highlighting the bifurcation of multi-potent progenitor cells (MPP) cells into common lymphoid progenitor cells (CLP) or common myeloid progenitor cells (CMP). HSC indicates hematopoietic stem cells. F) HSC and MPP cells from bone marrow in A) were counted as in C). Results from the different genotypes are not significantly different (t-test P>0.18). G) Data from E) is plotted as a percentage of total viable bone marrow cells analyzed. Significant differences (t-test P<0.05) between genotypes are marked by *.