Plant-Generated Artificial Small RNAs Mediated Aphid Resistance
Figure 3
Expression levels of artificial microRNAs and small interfering RNAs, targeting the MpAChE2 gene of Myzus persicae.
Relative expression levels of amiR-AChE2-1 (A) or amiR-AChE2-2 (B) in tobacco plants analyzed by qRT-PCR. The expression levels of amiRNAs were relative to the expression of tobacco 5.8S rRNA and normalized. The data represent the means (±SE) of three biological replicates. Student’s t-test was used to determine significant differences in transgenic plants compared to wild type plants (An asterisk denotes p<0.01, and a double asterisk indicates p<0.001). (C) Northern blot detection of AChE2 siRNAs in plants. rRNA/tRNA stained with ethidium bromide was used as a loading control.