Broad T-Cell Receptor Repertoire in T-Lymphocytes Derived from Human Induced Pluripotent Stem Cells
Figure 2
Generation of T lymphocytes from hiPSC and hESC (H1).
Human iPSC- and hESC-derived CD34+ cells were purified on anti-CD34+ magnetic beads after a two-step, 18-day co-culture with OP9 cells and subsequently plated on OP9-DL4 stromal cells. After 14, 21, 28, and 35 days of co-culture, the cells were analyzed for the indicated T cell markers. a, CD4, CD8. b,TCR-γδ and surface CD3. c,TCR-αβ and surface CD3. Similar data for hiPSC-19 are illustrated in Figure S10).