Skip to main content
Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

Sequencing and Validation of Reference Genes to Analyze Endogenous Gene Expression and Quantify Yellow Dwarf Viruses Using RT-qPCR in Viruliferous Rhopalosiphum padi

Figure 7

Mean relative expression values (± SE, n = 3) for endogenous aphid genes ago-1a-1 (A), ago-1a-2 (B), ago-1a-3 (C) and dcr1 (D) in BYDV-GPV-viruliferous winged adults of Rhopalosiphum padi after different virus-feeding durations.

A–D, Expression values for each gene or each region of one gene at each duration were normalized with the reference gene(s) selected by GeNorm and then compared with a one-way ANOVA (p) among these durations with each normalization condition. 2re = 2 best reference genes; 3re = 3 best reference genes; 4re = all 4 reference genes; bad2re = 2 least stable reference genes; EF-1α = EF-1α as the reference gene; 18S = 18S rRNA as the reference gene.

Figure 7

doi: https://doi.org/10.1371/journal.pone.0097038.g007