Quantification of Blood Flow and Topology in Developing Vascular Networks
Figure 3
For the micro-PIV measurements in the vitelline network, the embryo is placed in a bath of constant temperature of 38°C under the microscope.
The vitelline network is clearly visible through the microscope since the network and embryo are floating on top of the egg yolk. A high-powered LED illuminates the tissue directly, not through the microscope.