Variants of a Thermus aquaticus DNA Polymerase with Increased Selectivity for Applications in Allele- and Methylation-Specific Amplification
Figure 1
Investigated mutation sites in KlenTaq.
A) Crystal structure view showing the active ternary complex of KlenTaq. The enlarged sections show the interaction of the amino acid of interest with the phosphate of the primer backbone (PDB-code 3KTQ[68]). B) 2D-schematic drawing of the DNA substrate, template (left) and primer (right). Arrows indicate the interaction of the amino acids according to [68].