Cardiomyocyte-Specific miRNA-30c Over-Expression Causes Dilated Cardiomyopathy
Figure 3
Histological analysis of miRNA-30c transgenic mice.
(a) Hematoxylin-Azophloxin (HA) and sirius red staining of end-stage failing heart showing ventricular dilation and enlarged right atria in combination with interstitial fibrosis during the final stage of disease in line A. (b) Representative images of cardiac sections stained with Sirius Red show no difference in interstitial fibrosis between wildtype and transgenic mice, at 12 weeks of age in line A. (c) Quantification of interstitial fibrosis for wildtype and miRNA-30c TG mice (N≥5, 20 images per heart). (d) CTGF mRNA expression, corrected for GAPDH, at several ages in line B as evaluated by qPCR (N≥3). (e) Quantification of the cardiac area, Nkx2.5-positive nuclei and cell volumes at 4 weeks of age in line B. Quantification is based on stitched images of whole cardiac sections as shown in panel of B and Figure S4 (N≥5). Error bars represent s.e.m. and * denotes a p-value ≤0.05.