TAT-Protein Blockade during Ischemia/Reperfusion Reveals Critical Role for p85 PI3K-PTEN Interaction in Cardiomyocyte Injury
Figure 4
Effect of TAT-ΔPTEN p85 on Akt activation and cardiomyocyte injury caused by I/R.
A. Cardiomyocytes were added with 1.7 µM TAT-ΔPTEN p85 or 1.7 µM TAT-GFP, prior to simulated ischemia for 90 min followed by 30 min reperfusion (I/R) at 37°C. Expression and phosphorylation of Akt in cardiomyocytes was detected by specific antibody. The values presented were the mean ± SEM from 3 independent experiments. B. Cardiomyocytes were added with 1.7 µM TAT-ΔPTEN p85 or 1.7 µM TAT-GFP, prior to simulated ischemia for 90 min followed by 3 h reperfusion (I/R) at 37°C. Cardiomyocyte injury was measured by propidium iodide staining. The values presented were the mean ± SEM from 3 independent experiments. *p<0.01 vs. I/R alone.