Development of a Chemically Defined Medium and Discovery of New Mitogenic Growth Factors for Mouse Hepatocytes: Mitogenic Effects of FGF1/2 and PDGF
Figure 5
Rat hepatocyte proliferation in the MHGM medium.
Y-axis indicates percent of labeled nuclei due to incorporation of bromodeoxyuridine into DNA. The scale of Y-axis is kept identical to that of figure 4, for direct comparison of the responses of mouse and rat hepatocytes. The growth factors added to the medium are listed under the X-axis. CTR: Control cultures, with no growth factors added. Growth factors were added at 24 hours after plating of hepatocytes. D 0–2 (etc.) indicates days in culture after addition of the growth factors. (For growth factor concentration, please see Materials and Methods).