Quantitative-Proteomic Comparison of Alpha and Beta Cells to Uncover Novel Targets for Lineage Reprogramming
Figure 1
Experimental design for comparative analyses of pancreatic alpha and beta cells using chemical probes, gene-expression analysis, and mass spectrometry-based quantitative phosphoproteomics.
(A) Computational prediction and experimental validation of phenotypic differences between mouse alpha cells (αTC1) and beta cells (βTC3) using comparative gene-expression analysis. (B) Workflow for quantitative proteomic and phosphoproteomic analysis of alpha and beta cells, and alpha cells treated with BRD7389 and GW8510. (C) Outline of SILAC conditions upon which quantification was performed. These analyses generated a candidate list of kinases differentially expressed in alpha and beta cells from which target kinases were identified using knockdown, chemical genetics, and immunocytochemistry.