Palmitoylation of the Immunity Related GTPase, Irgm1: Impact on Membrane Localization and Ability to Promote Mitochondrial Fission
Figure 4
Effect of 2-bromopalmitate on Irgm1 association with the Golgi.
WT MEF were exposed to 100/ml IFN-γ for 24 h, followed by exposure to the palmitoylation inhibitor, 2-bromopalmitate (0.1 mM), for 2 h. The cells were then co-stained with anti-Irgm1, and (A) anti-GM130 or (B) anti-Tom20 antibodies, and used for immunofluorescence analysis. Shown is the overlap between the Irgm1 and GM130 signals as determined by colocalization analysis, and expressed as an average from three (GM130) or four (Tom20) separate experiments, with between 25 and 59 cells analyzed per group in each experiment. The error bars represent standard error of the mean. The effect of 2-BP was significant in (A) (p = 0.009), but not (B) (p = 0.066).