Development of a Highly Sensitive Loop-Mediated Isothermal Amplification (LAMP) Method for the Detection of Loa loa
Figure 3
Specificity assessment of LAMP assay.
(A) Visual examination of LAMP products by adding SYBR Green I. (B) Agarose gel electrophoresis of amplified products. Lanes Loa, Bp, Mp, Sv, Sm, Sh, Si, Sb, Fh, Ec, As, Dd, Hd, Tt, Eg: DNA from Loa loa, Brugia pahangi, Mansonella perstans, Strongyloides venezuelensis, Schistosoma mansoni, Schistosoma haematobium, Schistosoma intercalatum, Schistosoma bovis, Fasciola hepatica, Echinostoma caproni, Anisakis simplex, Dicrocoelium dendriticum, Hymenolepis diminuta, Taenia taeniformis and Echinococcus granulosus, respectively. Lane M: 50 bp DNA ladder (Molecular weight marker XIII, Roche); lane N: negative control (no DNA template).