The Mechanism of Poly-Galloyl-Glucoses Preventing Influenza A Virus Entry into Host Cells
Figure 3
pGG analogs directly bind and aggregate HA molecules.
(a) SPR results of the associations and dissociations of pGG analogs and HA molecules. Ka and Kd were calculated by fitting the binding model (Langmuir) to sensorgram curves using ProteOn Manager Software. (c-f) Native PAGE and silver stains reveal the effects of binding of PGG on the mobility of HA after pre-incubation with or without PGG. Four subtypes of purified proteins of HA (A/Puerto Rico/8/1934 (H1N1), A/California/04/2009 (H1N1), A/Netherlands/219/03 (H7N7), and A/Anhui/1/2005 (H5N1)) were tested and shown as panels (b), (c), (d), and (e), respectively.