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3D Non-Woven Polyvinylidene Fluoride Scaffolds: Fibre Cross Section and Texturizing Patterns Have Impact on Growth of Mesenchymal Stromal Cells

Figure 3

Proliferation rates of MSCs in 3D-PVDF non-wovens.

Effect of fibre shape on cell growth was assessed with the Alamar Blue assay 1, 5, 10, and 15 days after seeding. Cells in non-wovens with round or trilobal fibres displayed higher proliferations rates at day 10 and 15. Diagram bars represent the mean value of non-wowens with 10, 16 and 28 needles/inch texturizing (A). Effect of different texturizing on cell growth: cells in non-wovens with 10 needles/inch texturizing resulted in the highest proliferation rates at day 5, 10 and 15. Diagram bars represent the mean value of non-wowens with round, trilobal or snowflake fibre shape (B; n = 3; *p<0.05; **p<0.005; ***p<0.0005).

Figure 3

doi: https://doi.org/10.1371/journal.pone.0094353.g003