A Flow Cytometry-Based Quantitative Drug Sensitivity Assay for All Plasmodium falciparum Gametocyte Stages
Figure 3
Establishment of the FCM method for GFP detection in gametocytes.
(A) Scatter plots showing the gating signals of GFP-positive stage I–V gametocytes. Counting of gametocytes was done at relatively higher gametocytemias (%). Similar results were obtained when FCM counting of gametocytes were done at ∼0.02% gametocytemia (Figure S2 in File S1). (B) Uninfected RBCs (uRBC); (C) Asexual stages and (D) Gametocytes of the parental line 3D7 as negative controls. None of these controls emitted green fluorescence in the FITC channel. (E) Fluorescence intensity (FI) of gametocytes at different developmental stages.