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Celastrol Blocks Interleukin-6 Gene Expression via Downregulation of NF-κB in Prostate Carcinoma Cells

Figure 1

Celastrol inhibits PC-3 cell growth through cell cycle arrest at G0/G1 and apoptosis induction.

(A) PC-3 cells were treated with indicated concentrations of celastrol for 48 hours and the cell proliferation was determined by the H3-thymidine incorporation. (B) PC-3 cells were treated with indicated concentrations of celastrol for48 hours. Cells were lysed and expressions of PARP, cleaved PARP (c-PARP) were determined by immunoblotting assay. (C) After one day of treatment, the apoptotic index of PC-3 cells treated with different concentrations of celastrol was calculated. Each value is a mean ± SE of 3 determinations. (D) PC-3 cells were serum starved for 24 hours and then were treated with 0 – 1 μM of celastrol as indicated for 48 hours. The cells were stained with PI, and the cell cycle distribution was analyzed by flow cytometry. Each box represents the mean ± SE (n = 6). (* p<0.05; **p<0.01).

Figure 1

doi: https://doi.org/10.1371/journal.pone.0093151.g001