Tools for Gene-Regulatory Analyses in the Marine Annelid Platynereis dumerilii
Figure 1
Excision of a microinjected Tol2-based construct.
(A) Scheme of donor vector pTol2{rps9::egfp}frkt868; the rps::egfp cassette is flanked by inverted Tol2 repeats (Tol2IR) that serve as recognition site for Tol2 transposase co-injected as mRNA along with the vector DNA. (B, C) PCR using vector-specific primers (black arrows) yields a 200 bp PCR fragment specifically from embryos co-injected with tol2 transposase mRNA (left lane), but not from controls (right lane). (D) Precise cleavage of the reporter construct at the end of the Tol2 IRs is evidenced by sequencing of the 200 bp fragment.