Depletion of White Adipose Tissue in Cancer Cachexia Syndrome Is Associated with Inflammatory Signaling and Disrupted Circadian Regulation
Figure 6
Involvement of AMPK and mTOR/4EBP1 signaling pathways in cancer cachexia.
(A) mRNA analysis of Ampkα1, Ampkα2 and Acc from WAT of control (white circle), and C26-bearing mice (black square); (B) Western blot analysis of phospho-AMPK (Thr192) and total AMPK at 2 pm and 2 am; Immunoblot analysis of Phospho-ACC and total ACC for 2 pm WAT samples. (C) mRNA analysis of mTor and 4Ebp1; from WAT of control (white circle), and C26-bearing mice (black square); (D) Western blot analysis of phospho-mTOR (Ser2448) total mTOR, phospho-4EBP1 (Ser65), phospho-4EBP1 (Thr37/46) and total 4EBP1 at 2 pm and 2 am. Values are mean ± s.e.m. presented as percentages relative to the controls for 4–5 animals per group (*P<0.05; **P<0.01; C26 vs control). 6 am values are duplicated in each graph to illustrate a complete 24-h cycle.