Insulin-Like Growth Factor 1 Receptor (IGF-1R) as a Target of MiR-497 and Plasma IGF-1R Levels Associated with TNM Stage of Pancreatic Cancer
Figure 3
MiR-497 promoted chemosensitivity of gemcitabine.
(A) AsPC-1 cells were transfected for 24 hours, and then treated with 100nM gemcitabine for 48 hours. Upregulation of miR-497 by transfection of mimics re-sensitized cells to gemcitabine. Downregulation of miR-497 by transfection of inhibitor decreased the sensitivity of cells to gemcitabine. (B) BxPC-3 cells were transfected for 24 hours, and treated with serially diluted gemcitabine (1nM, 10nM, 30nM, 100nM). Cells transfected with miR-497 mimics were more sensitive to gemcitabine. (C) Cells transfected with miR-497 inhibitor were more resistant to gemcitabine. (* P<0.05).