Beneficial Metabolic Effects of Rapamycin Are Associated with Enhanced Regulatory Cells in Diet-Induced Obese Mice
Figure 2
(A) Representative sections of H&E-stained VWAT of Ve- or Rapa-treated mice. Scale bars represent 100 µm. Black arrows target to infiltrating cells. (B) Adipocyte diameter distribution of the VWAT of 5 Ve-treated and 5 Rapa-treated HFD-fed mice (Rapa: ▪, Ve: □). (C) RT-qPCR analysis of VWAT, after 22 injections (Rapa: ▪, Ve: □): Expression levels of T-cell (Cd4, Cd8, FoxP3), B-cell (Cd20) and macrophage (Cd68, F4/80) specific markers and of leukocyte migratory factors (Icam-1, Mcp-1) specific markers (normalized to Eef2 expression). Data are expressed as mean ± S.E.M. of 8 to 10 mice per group. #p<0.05, ##p<0.01. (D) Representative sections of the VWAT from Ve- or Rapa-treated mice immunostained with F4/80 Ab (brown color). Scale bars represent 100 µm (left). Quantification of F4/80 positive signal on VWAT sections by Image J (Rapa: ▪, Ve: □) (right). Data are expressed as mean ± S.E.M. of 5 mice per group. ##p<0.01.