Analysis of Autofluorescence in Polymorphonuclear Neutrophils: A New Tool for Early Infection Diagnosis
Figure 5
Fluorescence intensity and histology images of BAL in control and Pseudomonas aeruginosa- or Staphylococcus aureus–induced pneumonia mice.
Control condition histology (A) and fluorescence intensity imaging (D); Staphylococcus aureus-induced pneumonia histology (B) and fluorescence intensity imaging (E); Pseudomonas aeruginosa-induced pneumonia histology (C) and fluorescence intensity imaging (F). Scale bar, 50 μm. (G) Boxplot representation of fluorescence intensity in arbitrary units (I (AU)) of BAL for non infected mice (control, C) and Pseudomonas aeruginosa (PA) or Staphylococcus aureus (SA) induced pneumonia in mice. Boxes represent median (interquartile range). Data are representative of two independent experiments (Control group, n = 8; Pseudomonas aeruginosa group, n = 9; Staphylococcus aureus group, n = 12). ANOVA with post-hoc analysis by t-test using Bonferroni's correction was used to compare autofluorescence levels between each group. *: p<10−4 Pseudomonas aeruginosa and Staphylococcus aureus groups versus control group. &: p<10−4 Pseudomonas aeruginosa group versus Staphylococcus aureus group. BAL and background noise intensities are different in each condition (p<10−4). (H) Receiver operating curve of fluorescence intensity of cells between controls and induced-infectious pneumonia in mice. AUC ± Standard Error: area under curve. *p<10−4.