Enhanced Specificity of TPMT*2 Genotyping Using Unidirectional Wild-Type and Mutant Allele-Specific Scorpion Primers in a Single Tube
Figure 2
Construction of QC plasmid used in CRAS-PCR.
Panel A shows the PCR products of TPMT exon 4 containing the TPMT*2 locus at a 55 to 65°C gradient annealing temperature (lane 1 to 8). Panels B and C show the sequencing chromatogram images of WT- and MT-QC plasmid, in which the arrows indicate the location of TPMT*2, and the underlined bases show the codon containing TPMT*2.