The Cellular Distribution and Ser262 Phosphorylation of Tau Protein Are Regulated by BDNF In Vitro
Figure 5
Tau protein distribution in DIV4 nocodazole-treated E18 primary hippocampal neurons.
(A) Tau protein cellular distribution in control primary cultured hippocampal neurons. Arrow indicates the tau staining in the soma. (B) Example of nocodazole-damaged neuron. The tau protein signal (red) in the somatic compartment was much stronger than the Hoechst signal from the nuclei (blue), resulting in the merged purple in the soma as shown by arrow in the merged column. (C) Example of nocodazole-damaged neuron treated with BDNF. BDNF treatment decreased tau immunoreactivity in the soma compare to the nocodazole-treated group. Arrow indicates the tau staining in the soma (Bar = 20 μm). (D) Average neurite length of neurons in each group (*p<0.05, ***p<0.001).