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Biochemical and Structural Characterization of Neocartilage Formed by Mesenchymal Stem Cells in Alginate Hydrogels

Figure 2

IHC staining of type II collagen (red), type II procollagen (red), ADAMTS-2 (green), ADAMTS-3 (green) and BMP-1 (green), counter-stained with DAPI (blue).

In type II collagen images, pan cadherin was also stained (green). Original images of type II procollagen were captured by optimizing for signal in the ECM (I0) and the shown images were further enhanced according to . Insets show low magnification images of the respective samples. Scale bars are 50 µm.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0091662.g002