The Tumor Suppressor Role of miR-124 in Osteosarcoma
Figure 4
Rac1 is a direct target of miR-124.
(A) Predicted duplex formation between human Rac1 3′-UTR and miR-124, Rac1 3′-UTR is highly conserved in different species. Upper panel, sequence alignment of miR-124 with binding site on the Rac1 3′-UTR. Lower panel, sequence of the miR-124 binding site within the Rac1 3′-UTR of four species. (B) MiR-124 cannot alter mRNA level of Rac1 by real-time PCR. (C) Luciferase activity of wild-type (WT-UTR) or mutant (MUT-UTR). (D) Rac1 protein expression in MG-63 cells were transfected with 50 nmol/L of miR-124 mimics , sramble or not transfected.