Restrictive Expression of Acid-Sensing Ion Channel 5 (Asic5) in Unipolar Brush Cells of the Vestibulocerebellum
Figure 1
The Asic5tm2a(KOMP)Wtsi mouse.
A. A simplified scheme showing the wild type Asic5 (top) and reporter Asic5tm2a(KOMP)Wtsi (bottom) alleles as well as the orientation and relative positions of binding sites for primers used in genotyping reactions. The neo gene in the trapping cassette is not shown in this figure. B. Inverted images of representative gels (n≥60) containing typical products from genotyping reactions of homozygous wild type and Asic5tm2a(KOMP)Wtsi reporter mice using primers 1, 2 & 3. Expected products are indicated with arrowheads. C. Inverted image of a representative gel (n>3) containing the products of long range PCR reactions across both insertion arms. D. Inverted images of representative gels containing typical RT-PCR products generated from mRNA isolated from the cerebellum (left) and liver (right) of Asic5tm2a(KOMP)Wtsi mice and wild type littermates. Primers specific for lacZ and actin mRNAs were used in these reactions with actin serving as a positive control. Arrowheads indicate the expected lacZ product.