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Sterol Regulatory Element-Binding Proteins Are Regulators of the Rat Thyroid Peroxidase Gene in Thyroid Cells

Figure 4

In vitro-binding of nuclear SREBP-1c and SREBP-2 to the first intron of the rat TPO gene.

(A) Schematic representation of the overlapping oligonucleotide fragments corresponding to the intronic nucleotide sequence of the rat TPO gene between +598 to +697. Positions are indicated relative to the transcription start site. (B, C) In vitro-binding of rat nuclear SREBP-1c (B) and rat nuclear SREBP-2 (C) to different sequences of the first intron of the rat TPO gene (rTPO I–VI). EMSA was performed using in vitro-translated rat nuclear SREBP-1c or rat nuclear SREBP-2 and DIG-labeled oligonucleotide spanning the intronic nucleotide sequence of the rat TPO gene from +598 to +697. The use of DIG-labeled specific probe (human LDLR-SRE) and non-specific probe (mutated human LDLR-SRE) is also indicated. For illustrating unspecific bands, in vitro-translated pcDNA3.1 was incubated with DIG-labeled oligonucleotide corresponding to rTPO III (lane 10).

Figure 4

doi: https://doi.org/10.1371/journal.pone.0091265.g004