High-Throughput Screening Reveals Alsterpaullone, 2-Cyanoethyl as a Potent p27Kip1 Transcriptional Inhibitor
Figure 1
Design and validation of a high throughput screen to antagonize p27Kip1 transcription in HeLa cells.
A. Workflow for the primary screen. B. Schematic of the 4-kb human p27Kip1 promoter driven luciferase plasmid used in this study. C. Performance of the p27Kip1-luciferse assay against negative controls (DMSO, green triangle) and titrating amounts of the positive control (cyclohexamide, black circle). D. Z’ factor calculations for assay performance over 34 plates from the primary screen. Mean Z’ factor = 0.74±0.06.