Heterologous Protein Secretion in Lactobacilli with Modified pSIP Vectors
Figure 2
Growth curves for induced (–) and non-induced (–) cells of five different Lactobacillus species containing pLp3050NucA-SH71.
Optical density (OD600) was measured every hour after induction of NucA production. The graphs show L. plantarum (▴), L. gasseri (□), L. rhamnosus (▪), L. curvatus (•), and L. brevis (○). For comparison, graphs for L. plantarum harboring pLp3050NucA (×) (256rep) are also shown. L. curvatus and L. brevis species were grown at 30°C, whereas the other species were grown at 37°C.