Moraxella catarrhalis Adhesin UspA1-derived Recombinant Fragment rD-7 Induces Monocyte Differentiation to CD14+CD206+ Phenotype
Figure 1
CD206, CD80 and CD86 expression on CD14+ cells after stimulation with rD-7 and r6–8.
(A) 1×106 monocytes were treated with rD-7 (2.5 µg/ml), r6–8 (2.5 µg/ml) or LPS (2 µg/ml) for 24 h, cells collected and live cells sorted using the vital dye eFluor 780. CD14+ cells were then gated for the analysis of CD206, CD80 and CD86 expression by flow cytometry. Black and grey histograms represent stained cells and unstained cells respectively. (B) The MFI of CD14+ cells expressing CD206, CD80 or CD86 are shown. rD-7, r6–8 or LPS was used in three independent experiments at least and each dot represents a separate experiment (*, P<0.05; ***, P<0.001).