Expression Level and Subcellular Localization of Heme Oxygenase-1 Modulates Its Cytoprotective Properties in Response to Lung Injury: A Mouse Model
Figure 1
Expression, localization, and HO activity of HA-tagged HO-1 protein in the murine lung.
(a) Human SP-C/trans-modified vector construct. (b) HA-tagged HO-1 cDNA constructs. Numbers denote amino acids in the fragment starting from the N terminus. NLS, nuclear localization sequence. (c) Immunoblots showing lung-specific overexpression of high or low levels of HA-tagged HO-1-FL (FL(H) or FL(L)) or Nuc-HO-1-TR (TR). Membranes were re-probed with calnexin antibodies as a loading control. (d) HA immunostaining (green) and nuclear DAPI staining (blue) in lung slices from HO-1-FL (FL) and Nuc-HO-1-TR (TR) transgenic mice. (e) Total lung HO activity in whole lung homogenates on day 14 of life. Values are the mean ± SEM of 3 separate determinations in each group. *, p<0.05 vs WT.